FRAP Analysis of Membrane-Associated Proteins: Lateral Diffusion and Membrane-Cytoplasmic Exchange
نویسندگان
چکیده
منابع مشابه
FRAP analysis of membrane-associated proteins: lateral diffusion and membrane-cytoplasmic exchange.
Obtaining quantitative kinetic parameters from fluorescence recovery after photobleaching (FRAP) experiments generally requires a theoretical analysis of protein mobility and appropriate solutions for FRAP recovery derived for a given geometry. Here we provide a treatment of FRAP recovery for a molecule undergoing a combined process of reversible membrane association and lateral diffusion on th...
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We present a method describing the lateral movement of proteins in cell membranes as observed in FRAP experiments. We extend earlier results derived for normal diffusion [1] to account for the case of anomalous subdiffusion. Our analytic closed forms are compared to computer simulations of anomalous diffusion and both show excellent agreement. The approach sheds light on the behavior of protein...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.08.033